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Title
Simple Cell Imaging for Complex Research
Monday, September 21, 2015
Veronika Kortisova Descamps
The average rat brain is about the size of a pea and is divided into more than a dozen functional structures.
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Single-Cell RNA Detection to Identify Cell Subpopulations in Heterogeneous Mixtures
Tuesday, March 25, 2014
Don Weldon
Victor Koong
Alex Ko
Yuko Williams
Laura White
Traditional RNA detection methods, which analyze RNA levels in lysed, permeabilized, or fixed cell samples, make it challenging to understand the role of one gene in the context of a tissue, organ, or...
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siRNA Knockdown: Implementing Negative Controls in Antibody QC
Friday, January 20, 2017
Will Olds
This article presents an overview of short interfering RNA (siRNA) knockdown, a powerful negative control method, followed by tips to optimize siRNA transfection experiments.
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Smart Sample Prep in Chromatography: An Automated Liquid–Liquid Extraction Technique for Metabolomics Analysis
Friday, February 19, 2016
Emmanuel Varesio
Sandra Jahn
Sandrine Cudré
Gérard Hopfgartner
Renzo Picenoni
Guenter Boehm
As laboratories continue to be asked to increase productivity and the analysis power that is available with advanced chromatography systems continues to grow, the focus for many scientists is firmly ...
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Super-Resolution Microscopy Helps Extend Optical Observation Beyond Its Theoretical Boundaries
Wednesday, March 09, 2016
Russell Ulbrich
Scientists have long needed to view structures and processes that cannot be resolved via traditional optical microscopy.
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Super-Resolution Microscopy Techniques Combine to Clarify Spatiotemporal Structure of the Endoplasmic Reticulum
Friday, February 17, 2017
Robert L. Stevenson, Ph.D.
Super-resolution microscopy encompasses several new techniques for direct spatiotemporal imaging of structural features much smaller than the diffraction limit of light. The endoplasmic reticulum (ER)...
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Ten Tips for Proper Pipetting
Monday, November 07, 2016
A. Björn Carle
Pipetting proficiency is one of the most critical elements to ensure accuracy and precision, particularly in the face of high personnel turnover, growing laboratory workloads and increasingly complex ...
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TERS—Ready or Not?
Thursday, September 01, 2016
Barbara Foster
Tip-enhanced Raman spectroscopy, or TERS, integrates scanning probe microscopy (SPM) for nano-scale imaging with confocal Raman spectroscopy for chemical characterization.
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The Benefits of HPLC Coupled With Mass Spectrometry for the Direct Quantitation of Underivatized Amino Acids
Monday, April 04, 2016
Chi Man Ng
Wilhad M. Reuter
Amino acids are critical biological components having multiple metabolic functions, particularly when linked together to form proteins, and have any number of important roles.
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The Future of DNA Synthesis
Thursday, May 26, 2016
Devin Leake
Since the launch of the Human Genome Project, the genomes of over 180 organisms have been sequenced.
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The Road to Improved Next-Generation Sequencing Through Innovative PCR Instrumentation
Friday, November 20, 2015
Andrew Birnie
Next-generation sequencing (NGS), one of the most significant technological advances in the the last 30 years, allows millions of DNA strands to be sequenced in parallel.
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The Scales of Scientific Justice in Translational Science: Data Reproducibility and Verifiable Science
Tuesday, March 04, 2014
Tristan Berto
The traditional “scales of justice” represent truth and fairness. For the scientific community, the balance is between big data (terabytes of data across numerous databases) on one scale and ...
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The Science of a Lab Relocation
Thursday, June 20, 2019
George Rohlfing
Key points to ensure a successful lab relocation.
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Top-Down Proteomics: An Emerging Technology Propelled by Mass Spectrometry
Wednesday, July 26, 2017
Rohan A. Thakur
Rapid developments in mass spectrometry technology facilitate innovative solutions to the challenges in top-down proteomics.
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Transitioning From HPLC to UHPLC of Proteins
Wednesday, March 09, 2016
Robert L. Stevenson, Ph.D.
Since the introduction of ACQUITY UPLC (Ultra Performance Liquid Chromatography) by Waters (Milford, Mass.) over a decade ago, there has been increased interest in the effects of high pressure on ...
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True Hypoxia Replication: Creating Optimal Conditions for Cell-Based Research
Monday, November 10, 2014
Michael Rau
There is a rapidly growing need for systems capable of creating precise, reproducible, and physiologically relevant cell environments in the laboratory in order to enable accurate analysis of both ...
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Use of Heat Stabilization to Preserve Lipids and Improve Sample Quality
Friday, June 12, 2015
Marcus Söderquist
Lipids have long been considered to be mere building blocks of cell membranes or energy-storage molecules. It is now evident, however, that these small, nonpolymeric molecules play several key roles ...
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Validation of a Magnetic Bead Mixer on an Automated Next-Generation Sequencing Library Preparation System
Tuesday, April 07, 2015
Sikander Gill
Rajwant Gill
Nick Bandy
Dong Liang
Reduced costs and higher throughput have made next-generation sequencing (NGS) more accessible to users in such diverse fields as diagnostics, infectious disease, food safety and public health. NGS ...
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Wyatt’s International Light Scattering Colloquium Focuses on the Nano World
Wednesday, January 27, 2016
Robert L. Stevenson, Ph.D.
The 24th International Light Scattering Colloquium, which attracted about 70 scientists to the Biltmore Hotel on Butterfly Beach in Santa Barbara, Calif., November 3–4, focused on analytical ...
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